Visualization 1. Planar substrates.mp4

This video demonstrates the plasmo-thermal E. coli accumulation effect on planar substrates under a Raman imaging microscope (DXR2xi, Thermo Fisher Scientific, USA). Data was acquired using a 785 nm excitation laser, a 50 µm slit aperture, a 50× long working distance objective (Olympus, Japan), 5 mW of excitation power, a 2.5 ms (400 Hz) excitation time, and 10 averaging scans. The microscope’s white light illumination mode was used to record the video clips. These were taken using a screen recording software (Bandicam, Bandicam Company, USA) before and after 4 minutes of laser exposure at a spot on a plain gold surface and on several hexagonal single voxel arrays (HSVAs) with spacing distances (a) of 300/700/800/1,100/1,400 nm. Due to the interlock mechanism of the imaging Raman microscope, live imaging was not available during laser exposure. The video starts from the plain gold surface and then shows the HSVAs in order of increasing spacing distance. The video clips of all the cases after the exposure are collated at the end of the video to allow intuitive comparison. The video demonstrates that the highest number of swimming E. coli were trapped/accumulated on the HSVA with a = 700 nm.