3D imaging in CUBIC-cleared mouse heart tissue: going deeper

Gómez-Gaviro, María Victoria

3D imaging in CUBIC-cleared mouse heart tissue: going deeper

Posted on 2017-04-26 - 17:42

The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.

CITE THIS COLLECTION

DataCite

3 Biotech

3D Printing in Medicine

3D Research

3D-Printed Materials and Systems

4OR

AAPG Bulletin

AAPS Open

AAPS PharmSciTech

Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg

ABI Technik (German)

Academic Medicine

Academic Pediatrics

Academic Psychiatry

Academic Questions

Academy of Management Discoveries

Academy of Management Journal

Academy of Management Learning and Education

Academy of Management Perspectives

Academy of Management Proceedings

Academy of Management Review

Nehrhoff, Imke; Bocancea, Diana; Vaquero, Javier; Vaquero, Juan José; Ripoll, Jorge; Desco, Manuel; et al. (2016). 3D imaging in CUBIC-cleared mouse heart tissue: going deeper. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3753443.v1

https://doi.org/10.6084/m9.figshare.c.3753443.v1

or

Select your citation style and then place your mouse over the citation text to select it.