Continuous focal translation enhances rate of point-scan volumetric microscopy
Posted on 2019-11-26 - 19:06
Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by moving a stage, this per-frame imaging process is time consuming. Here, we present a method for an easy-to-implement and inexpensive modification of an existing two-photon microscope to rapidly image in 3D using an electrically tunable lens to create a tessellating scan pattern which repeats with the volume rate. Using appropriate interpolating algorithms, the volumetric imaging rate can be increased by a factor up to four-fold. This capability provides the expansion of the two-photon microscope into the third dimension for faster volumetric imaging capable of visualizing dynamics on timescales not achievable by traditional stage-stack methods.
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Johnson, Courtney; Exell, Jack; Kuo, Jonathon; Welsher, Kevin (2019). Continuous focal translation enhances rate of point-scan volumetric microscopy. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4637714.v1
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AUTHORS (4)
CJ
Courtney Johnson
JE
Jack Exell
JK
Jonathon Kuo
KW
Kevin Welsher