Deep learning-based adaptive optics for light sheet fluorescence microscopy
Posted on 2023-05-25 - 15:51
Light sheet fluorescence microscopy (LSFM) is a high-speed imaging technique that is often used to image intact tissue-cleared specimens with cellular or subcellular resolution. Like other optical imaging systems, LSFM suffers from sample-induced optical aberrations that decrement imaging quality. Optical aberrations become more severe when imaging a few millimeters deep into tissue-cleared specimens, complicating subsequent analyses. Adaptive optics is commonly used to correct sample-induced aberrations using a deformable mirror. However, routinely used sensorless adaptive optics techniques are slow, as they require multiple images of the same region of interest to iteratively estimate the aberrations. In addition to the fading of fluorescent signal, this is a major limitation as thousands of images are required to image a single intact organ even without adaptive optics. Thus, a fast and accurate aberration estimation method is needed. Here, we used deep-learning techniques to estimate sample-induced aberrations from only two images of the same region of interest in cleared tissues. We show that application of correction using a deformable mirror greatly improves image quality. We also introduce a sampling technique that requires minimum number of images to train the network. Two conceptually different network architectures are compared; one that shares convolutional features, and another that estimates each aberration independently. Overall, we have presented an efficient way to correct aberrations in LSFM, and to improve image quality.
CITE THIS COLLECTION
Rai, Mani; Li, Chen; Ghashghaei, H; Greenbaum, Alon (2023). Deep learning-based adaptive optics for light sheet fluorescence microscopy. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.6639500.v2
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