Endoscopic en-face optical coherence tomography and fluorescence imaging using correlation-based probe tracking

Posted on 14.01.2022 - 16:44
Forward-viewing endoscopic optical coherence tomography (OCT) provides 3D imaging in vivo, and can be combined with widefield fluorescence imaging by use of a double-clad fiber. However, it is technically challenging to build a high-performance miniaturized 2D scanning system with a large field-of-view. In this paper we demonstrate how a 1D scanning probe, which produces cross-sectional OCT images (B-scans) and 1D fluorescence T-scans, can be transformed into a 2D scanning probe by manual scanning along the second axis. OCT volumes are assembled from the B-scans using speckle decorrelation measurements to estimate the out-of-plane motion along the manual scan direction. Motion within the plane of the B-scans is corrected using image registration by normalized cross correlation. En-face OCT slices and fluorescence images, corrected for probe motion in 3D, can be displayed in real-time during the scan. For a B-scan frame rate of 250 Hz, and an OCT lateral resolution of approximately 20 µm, the approach can handle out-of-plane motion at speeds of up to 4 mm/s.

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Marques, Manuel; Hughes, Michael; Uceda, Adrian Fernandez; Gelikonov, Grigory; Bradu, Adrian; Podoleanu, Adrian (2022): Endoscopic en-face optical coherence tomography and fluorescence imaging using correlation-based probe tracking. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.5651557.v2
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AUTHORS (6)

Manuel Marques
Michael Hughes
Adrian Fernandez Uceda
Grigory Gelikonov
Adrian Bradu
Adrian Podoleanu
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