Endoscopic en-face optical coherence
tomography and fluorescence imaging using
correlation-based probe tracking
Posted on 14.01.2022 - 16:44
Forward-viewing endoscopic optical coherence tomography (OCT) provides 3D
imaging in vivo, and can be combined with widefield fluorescence imaging by use of a double-clad
fiber. However, it is technically challenging to build a high-performance miniaturized 2D scanning
system with a large field-of-view. In this paper we demonstrate how a 1D scanning probe, which
produces cross-sectional OCT images (B-scans) and 1D fluorescence T-scans, can be transformed
into a 2D scanning probe by manual scanning along the second axis. OCT volumes are assembled
from the B-scans using speckle decorrelation measurements to estimate the out-of-plane motion
along the manual scan direction. Motion within the plane of the B-scans is corrected using
image registration by normalized cross correlation. En-face OCT slices and fluorescence images,
corrected for probe motion in 3D, can be displayed in real-time during the scan. For a B-scan
frame rate of 250 Hz, and an OCT lateral resolution of approximately 20 µm, the approach can
handle out-of-plane motion at speeds of up to 4 mm/s.
CITE THIS COLLECTION
Marques, Manuel; Hughes, Michael; Uceda, Adrian Fernandez; Gelikonov, Grigory; Bradu, Adrian; Podoleanu, Adrian (2022): Endoscopic en-face optical coherence
tomography and fluorescence imaging using
correlation-based probe tracking. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.5651557.v2
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AUTHORS (6)
MM
Manuel Marques
MH
Michael Hughes
AU
Adrian Fernandez Uceda
GG
Grigory Gelikonov
AB
Adrian Bradu
AP
Adrian Podoleanu