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Light-Field Microscopy with Temporal Focusing Multiphoton Illumination for Scanless Volumetric Bioimaging

Posted on 2022-11-23 - 14:50
A temporal focusing multiphoton illumination (TFMI) method is proposed for achieving selective volume illumination (SVI) (i.e., illuminating only the volume of interest) in light-field microscopy (LFM). The proposed method minimizes the background noise of the LFM images and enhances the contrast, and thus improves the imaging quality. Three-dimensional (3D) volumetric imaging is achieved by reconstructing the LFM images using a phase-space deconvolution algorithm. The experimental results obtained using 100-nm fluorescent beads show that the proposed TFMI-LFM system achieves lateral and axial resolutions of 1.2 μm and 1.1 μm, respectively, at the focal plane. Furthermore, the TFMI-LFM system enables 3D images of the single lobe of the drosophila mushroom body with GFP biomarker (OK-107) to be reconstructed in a one-snapshot record.

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Biomedical Optics Express

AUTHORS (6)

Feng-Chun Hsu
Chun-Yu Lin
Yvonne Hu
Yeukuang Hwu
Ann-Shyn Chiang
Shean-Jen Chen

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