Miniature Structured Illumination Microscope for in vivo 3D Imaging of Brain Structures with Optical Sectioning

Shepherd, Douglas

Miniature Structured Illumination Microscope for in vivo 3D Imaging of Brain Structures with Optical Sectioning

Version 2 2022-03-29, 13:12

Version 1 2022-03-29, 13:12

Posted on 2022-03-29 - 13:12

We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 μm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 μm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 μm in the brain.

CITE THIS COLLECTION

DataCite

3 Biotech

3D Printing in Medicine

3D Research

3D-Printed Materials and Systems

4OR

AAPG Bulletin

AAPS Open

AAPS PharmSciTech

Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg

ABI Technik (German)

Academic Medicine

Academic Pediatrics

Academic Psychiatry

Academic Questions

Academy of Management Discoveries

Academy of Management Journal

Academy of Management Learning and Education

Academy of Management Perspectives

Academy of Management Proceedings

Academy of Management Review

Supekar, Omkar; Sias, Andrew; Hansen, Sean; Martinez, Gabriel; Peet, Graham; Peng, Xiaoyu; et al. (2022). Miniature Structured Illumination Microscope for in vivo 3D Imaging of Brain Structures with Optical Sectioning. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.5721200.v2

https://doi.org/10.6084/m9.figshare.c.5721200.v2

or

Select your citation style and then place your mouse over the citation text to select it.