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Multi-wavelength quantitative polarization and phase microscope

Posted on 2019-03-05 - 19:49
We introduce a snapshot multi-wavelength quantitative polarization and phase microscope (MQPPM) for measuring the spectral dependent quantitative polarization and phase information. The system uniquely integrates a polarized light microscope and a snap-shot quantitative phase microscope in a single system, utilizing a novel full-Stokes camera operating in the red, green, and blue (RGB) spectrum. The linear retardance and fast axis orientation of birefringent sample can be measured simultaneously in the visible spectrums. Both theoretical analysis and experiment have been performed to demonstrate the principle of the proposed microscope. Data from liquid crystal and different biological samples are presented. We believe that MQPPM will be a useful tool in providing polarization and phase imaging of live cell dynamics.

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Biomedical Optics Express

AUTHORS (8)

Xiaobo Tian
Xingzhou Tu
Kimiko Croce
Guang Yao
Haijiang Cai
Neal Brock
Stanley Pau
Rongguang Liang

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