OCT based four-dimensional cardiac imaging of living chick embryo using impedance signal as the gating for post-acquisition synchronization

Posted on 22.11.2022 - 15:12
Four-dimensional (4-D) imaging of embryonic heart is valuable for the study of in vivo cardiac morphology and function during development. Optical coherence tomography (OCT) is a non-invasive imaging modality with high spatial resolution suitable for early embryonic heart imaging. However, most commonly used OCT systems cannot provide direct 4-D imaging due to acquisition speed limitations. We proposed a retrospective gating 4-D reconstruction method based on spectral domain OCT. A special circuit was designed to measure impedance change of chick embryo in response to the heart beating. The impedance signal was acquired simultaneously with OCT B-scan image sequence at several different location along the heart. The impedance signal was used as a gating for 4-D reconstruction. The reconstruction algorithm includes cardiac period calculation, interpolation from multi cardiac cycle image sequence into one cardiac cycle, and cardiac phase synchronization among different location of the heart. The synchronism of the impedance signal change with the heartbeat was verified. Using the proposed method, we reconstructed the cardiac outflow tract (OFT) of chick embryos at an early stage of development (Hamburger–Hamilton stage 18). We showed that the reconstructed 4-D images correctly captured the dynamics of the OFT wall motion.

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Ma, Yushu; Li, Chuanxi; Jiang, Huiwen; Zhao, Yuqian; LIU, JIAN; Yu, Yao; et al. (2022): OCT based four-dimensional cardiac imaging of living chick embryo using impedance signal as the gating for post-acquisition synchronization. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.6210949.v1
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AUTHORS (9)

Yushu Ma
Chuanxi Li
Huiwen Jiang
Yuqian Zhao
JIAN LIU
Yao Yu
Yi Wang
Wenbo Shi
Zhenhe Ma
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