Shot-noise limited tunable dual-vibrational frequency stimulated Raman scattering microscopy

Posted on 24.11.2021 - 15:55
We present a shot noise limited SRS implementation providing >200 mW per excitation wavelength that is optimized for addressing two molecular vibrations simultaneously. As the key to produce 3 ps laser of different color out of a single fs-laser (15 nm FWHM), we use ultra-steep angle-tunable optical filters to extract 2 narrow-band Stokes laser beams (1-2 nm & 1-2 ps) which are separated by 100 cm−1. The center part of the fs-laser is frequency doubled to pump an optical parametric oscillator (OPO). The temporal width of the OPO’s output (1 ps) is matched to the Stokes beams and can be tuned from 650-980 nm to address simultaneously two Raman shifts separated by 100 cm−1 that are located between 500 cm−1 and 5000 cm−1. We demonstrate background-free SRS imaging of C-D labeled biological samples (bacteria and Drosophila). Furthermore, high quality virtual stimulated Raman histology imaging of a brain adenocarcinoma is shown for pixel dwell times of 16 µs.

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Heuke, Sandro; Rimke, Ingo; Sarri, Barbara; Gasecka, Paulina; Appay, Romain; Legoff, Loic; et al. (2021): Shot-noise limited tunable dual-vibrational frequency stimulated Raman scattering microscopy. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.5667001.v2
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Biomedical Optics Express

AUTHORS (9)

Sandro Heuke
Ingo Rimke
Barbara Sarri
Paulina Gasecka
Romain Appay
Loic Legoff
Peter Volz
Edlef Büttner
Herve Rigneault

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