Optica Publishing Group
Browse

Super-Resolution Microscopy Based on the Inherent Fluctuations of Dye Molecules

Version 2 2025-02-04, 14:15
Version 1 2025-02-04, 14:15
Posted on 2025-02-04 - 14:15
Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization microscopy and stimulated-emission-depletion microscopy often demand considerable resources. These methods depend heavily on elaborate sample-staining, complex optical systems, or prolonged acquisition periods, and their application in 3D and multicolor imaging presents significant experimental challenges. In the current work, we provide a complete demonstration of a widely accessible super-resolution imaging approach capable of 3D and multicolor imaging based on super-resolution optical fluctuation imaging (SOFI). We replace the confocal pinhole with an array of single-photon avalanche diodes and use the microsecond-scale fluctuations of dye molecules as a contrast mechanism. This contrast is transformed into a super-resolved image using a robust and deterministic algorithm. Our technique utilizes natural fluctuations inherent to organic dyes, thereby it does not require engineering of the blinking statistics. Our robust, versatile super-resolution method opens the way to next-generation multimodal imaging and facilitates on-demand super-resolution within a confocal architecture.

CITE THIS COLLECTION

DataCite
No result found
or
Select your citation style and then place your mouse over the citation text to select it.

SHARE

email

Usage metrics

Biomedical Optics Express

AUTHORS (6)

Alexander Krupinski-Ptaszek
Adrian Makowski
Aleksandra Mielnicka
Monika Pawlowska
Ron Tenne
Radek Lapkiewicz

CATEGORIES

KEYWORDS

need help?