Super-Resolution Microscopy Based on the Inherent Fluctuations of Dye Molecules
Version 2 2025-02-04, 14:15Version 2 2025-02-04, 14:15
Version 1 2025-02-04, 14:15Version 1 2025-02-04, 14:15
Posted on 2025-02-04 - 14:15
Fluorescence microscopy is a critical tool across various disciplines, from materials
science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced
super-resolution techniques such as localization microscopy and stimulated-emission-depletion
microscopy often demand considerable resources. These methods depend heavily on elaborate
sample-staining, complex optical systems, or prolonged acquisition periods, and their application
in 3D and multicolor imaging presents significant experimental challenges. In the current
work, we provide a complete demonstration of a widely accessible super-resolution imaging
approach capable of 3D and multicolor imaging based on super-resolution optical fluctuation
imaging (SOFI). We replace the confocal pinhole with an array of single-photon avalanche diodes
and use the microsecond-scale fluctuations of dye molecules as a contrast mechanism. This
contrast is transformed into a super-resolved image using a robust and deterministic algorithm.
Our technique utilizes natural fluctuations inherent to organic dyes, thereby it does not require
engineering of the blinking statistics. Our robust, versatile super-resolution method opens the
way to next-generation multimodal imaging and facilitates on-demand super-resolution within a
confocal architecture.
CITE THIS COLLECTION
DataCite
DataCiteDataCite
No result found
Krupinski-Ptaszek, Alexander; Makowski, Adrian; Mielnicka, Aleksandra; Pawlowska, Monika; Tenne, Ron; Lapkiewicz, Radek (2025). Super-Resolution Microscopy Based on the Inherent Fluctuations of Dye Molecules. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.7564884.v2